pH-controlled recovery of placenta-derived mesenchymal stem cell sheets.
Identifieur interne : 001028 ( Main/Exploration ); précédent : 001027; suivant : 001029pH-controlled recovery of placenta-derived mesenchymal stem cell sheets.
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Abstract
Widely used in different biomedical applications, polyelectrolyte multilayers provide inter alia an attractive way for manufacturing of bio-functionalized, stimuli responsive surface coatings to control cellular behavior. In this study a novel polyelectrolyte-based platform for the engineering and controllable detachment of human mesenchymal stem cell (MSC) sheets is presented. Thin films obtained by layer-by-layer deposition of cationic poly(allylamine hydrochloride) (PAH) and anionic poly(styrene sulfonate) (PSS) polyelectrolytes on conductive indium tin oxide (ITO) electrodes allowed for the fast formation of viable sheets from human placenta-derived mesenchymal stem cells (PD-MSCs). Resulting stem cell sheets retained their phenotypical profile and mesodermal differentiation potency. Both electrochemically-induced local pH lowering and global decrease of the environmental pH allowed for a rapid detachment of intact stem cell sheets. The recovered stem cell sheets remained viable and maintained their capacity to differentiate toward the adipogenic and osteogenic lineages. This novel polyelectrolyte multilayer based platform represents a promising, novel approach for the engineering of human stem cell sheets desired for future clinical applications.
DOI: 10.1016/j.biomaterials.2011.02.058
PubMed: 21458856
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">pH-controlled recovery of placenta-derived mesenchymal stem cell sheets.</title><author><name sortKey="Guillaume Gentil, Orane" uniqKey="Guillaume Gentil O">Orane Guillaume-Gentil</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich</wicri:regionArea></affiliation></author><author><name sortKey="Semenov, Oleg V" uniqKey="Semenov O">Oleg V Semenov</name></author><author><name sortKey="Zisch, Andreas H" uniqKey="Zisch A">Andreas H Zisch</name></author><author><name sortKey="Zimmermann, Roland" uniqKey="Zimmermann R">Roland Zimmermann</name></author><author><name sortKey="V R S, Janos" uniqKey="V R S J">Janos Vörös</name></author><author><name sortKey="Ehrbar, Martin" uniqKey="Ehrbar M">Martin Ehrbar</name></author></titleStmt><publicationStmt><date when="2011">2011</date><idno type="doi">10.1016/j.biomaterials.2011.02.058</idno><idno type="RBID">pubmed:21458856</idno><idno type="pmid">21458856</idno><idno type="wicri:Area/Main/Corpus">001455</idno><idno type="wicri:Area/Main/Curation">001455</idno><idno type="wicri:Area/Main/Exploration">001028</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biocompatible Materials</term><term>Cell Culture Techniques (methods)</term><term>Cell Differentiation</term><term>Cells, Cultured</term><term>Electrochemical Techniques</term><term>Female</term><term>Humans</term><term>Hydrogen-Ion Concentration</term><term>Mesenchymal Stromal Cells (cytology)</term><term>Microscopy, Atomic Force</term><term>Placenta (cytology)</term><term>Pregnancy</term><term>Tissue Engineering (methods)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Biocompatible Materials</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Mesenchymal Stromal Cells</term><term>Placenta</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Cell Culture Techniques</term><term>Tissue Engineering</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Differentiation</term><term>Cells, Cultured</term><term>Electrochemical Techniques</term><term>Female</term><term>Humans</term><term>Hydrogen-Ion Concentration</term><term>Microscopy, Atomic Force</term><term>Pregnancy</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Widely used in different biomedical applications, polyelectrolyte multilayers provide inter alia an attractive way for manufacturing of bio-functionalized, stimuli responsive surface coatings to control cellular behavior. In this study a novel polyelectrolyte-based platform for the engineering and controllable detachment of human mesenchymal stem cell (MSC) sheets is presented. Thin films obtained by layer-by-layer deposition of cationic poly(allylamine hydrochloride) (PAH) and anionic poly(styrene sulfonate) (PSS) polyelectrolytes on conductive indium tin oxide (ITO) electrodes allowed for the fast formation of viable sheets from human placenta-derived mesenchymal stem cells (PD-MSCs). Resulting stem cell sheets retained their phenotypical profile and mesodermal differentiation potency. Both electrochemically-induced local pH lowering and global decrease of the environmental pH allowed for a rapid detachment of intact stem cell sheets. The recovered stem cell sheets remained viable and maintained their capacity to differentiate toward the adipogenic and osteogenic lineages. This novel polyelectrolyte multilayer based platform represents a promising, novel approach for the engineering of human stem cell sheets desired for future clinical applications.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">21458856</PMID><DateCreated><Year>2011</Year><Month>05</Month><Day>02</Day></DateCreated><DateCompleted><Year>2011</Year><Month>08</Month><Day>22</Day></DateCompleted><DateRevised><Year>2012</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1878-5905</ISSN><JournalIssue CitedMedium="Internet"><Volume>32</Volume><Issue>19</Issue><PubDate><Year>2011</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Biomaterials</Title><ISOAbbreviation>Biomaterials</ISOAbbreviation></Journal><ArticleTitle>pH-controlled recovery of placenta-derived mesenchymal stem cell sheets.</ArticleTitle><Pagination><MedlinePgn>4376-84</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.biomaterials.2011.02.058</ELocationID><Abstract><AbstractText>Widely used in different biomedical applications, polyelectrolyte multilayers provide inter alia an attractive way for manufacturing of bio-functionalized, stimuli responsive surface coatings to control cellular behavior. In this study a novel polyelectrolyte-based platform for the engineering and controllable detachment of human mesenchymal stem cell (MSC) sheets is presented. Thin films obtained by layer-by-layer deposition of cationic poly(allylamine hydrochloride) (PAH) and anionic poly(styrene sulfonate) (PSS) polyelectrolytes on conductive indium tin oxide (ITO) electrodes allowed for the fast formation of viable sheets from human placenta-derived mesenchymal stem cells (PD-MSCs). Resulting stem cell sheets retained their phenotypical profile and mesodermal differentiation potency. Both electrochemically-induced local pH lowering and global decrease of the environmental pH allowed for a rapid detachment of intact stem cell sheets. The recovered stem cell sheets remained viable and maintained their capacity to differentiate toward the adipogenic and osteogenic lineages. This novel polyelectrolyte multilayer based platform represents a promising, novel approach for the engineering of human stem cell sheets desired for future clinical applications.</AbstractText><CopyrightInformation>Copyright © 2011 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Guillaume-Gentil</LastName><ForeName>Orane</ForeName><Initials>O</Initials><Affiliation>Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, Switzerland.</Affiliation></Author><Author ValidYN="Y"><LastName>Semenov</LastName><ForeName>Oleg V</ForeName><Initials>OV</Initials></Author><Author ValidYN="Y"><LastName>Zisch</LastName><ForeName>Andreas H</ForeName><Initials>AH</Initials></Author><Author ValidYN="Y"><LastName>Zimmermann</LastName><ForeName>Roland</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Vörös</LastName><ForeName>Janos</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Ehrbar</LastName><ForeName>Martin</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Evaluation Studies</PublicationType><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>04</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biomaterials</MedlineTA><NlmUniqueID>8100316</NlmUniqueID><ISSNLinking>0142-9612</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Biocompatible Materials</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Biocompatible Materials</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cell Culture Techniques</DescriptorName><QualifierName MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cell Differentiation</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Electrochemical Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Hydrogen-Ion Concentration</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Mesenchymal Stromal Cells</DescriptorName><QualifierName MajorTopicYN="Y">cytology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Microscopy, Atomic Force</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Placenta</DescriptorName><QualifierName MajorTopicYN="Y">cytology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Pregnancy</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Tissue Engineering</DescriptorName><QualifierName MajorTopicYN="Y">methods</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year><Month>1</Month><Day>5</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2011</Year><Month>2</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2011</Year><Month>4</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>4</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>4</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>8</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pii">S0142-9612(11)00235-3</ArticleId><ArticleId IdType="doi">10.1016/j.biomaterials.2011.02.058</ArticleId><ArticleId IdType="pubmed">21458856</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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